Very pale lilac spore deposit.

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Image #8: Scoped w/ David Tate
Image #9: Scoped w/ David Tate
Image #10: Scoped w/ David Tate
Image #11: Scoped w/ David Tate

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Originally posted to Mushroom Observer on Oct. 18, 2017.

On herbaceous stems tiny yellows cup fungi.
Asci 8-spored, biseriate, IKI+r, croziers(+), 58x6um, 56x6um.
Ascospores subfusiform, aseptate, with few very small guttules, measured
*(9.1) 10.9 - 16.2 (17) × (1.7) 1.8 - 2.2 (2.5) µm
Q = (4.2) 4.9 - 7.9 (9.8) ; N = 23
Me = 12.9 × 2 µm ; Qe = 6.4
Paraphyses lanceolate, with 2-3 septa, with or without guttules.
Marginal hairs yellow-brown, cylindrical, septate with brown oil drops.

On a broken branch on the mossy ground in a mixed forest.
Anamorphic fungi. Growing superficially, nothing inside the bark.
Conidia hyaline (white in mass), aseptate, uninucleate, cylindrical, guttulate OCI=5, measured
*(17.9) 18.4 - 20.9 (22.4) × (7.7) 7.8 - 8.5 (8.6) µm
Q = (2.1) 2.2 - 2.5 (2.7) ; N = 12
Me = 19.6 × 8.2 µm ; Qe = 2.4

Tiny cup fungi on overwintered grass stems. Apothecia white, with smooth margin, stipitate. Asci 8-spored, H+.
Ascospores subfusiform, OCI=2.
Paraphyses cylindrical with highly refracting multiguttulate VBs.

Tiny cream color cup fungi on old grass stems.
Apothecia stipitate, with brownish hairs, 0.3-0.5mm in diameter.
Asci 8-spored, H-, IKI+b.
Ascospores narrow-fusiform, eguttulate, measured
*(11.7) 12.3 - 15.1 (16.9) × (2) 2.2 - 2.5 (2.6) µm
Q = (5) 5.4 - 6.6 (7.4) ; N = 26
Me = 13.9 × 2.3 µm ; Qe = 6
Paraphyses lanceolate, without VBs.
Ectal excipulum covered with brown cylindrical hairs. Hairs are warted.

Tiny black anamorphic fungi on decorticated log. Fruitbodies about 0.5mm in height, producing whitish conidia mass at the top.
Conidia aseptate, cylindrical to ellipsoid, measured
*(4.1) 4.2 - 5.1 (5.5) × 2 - 2.29 (2.3) µm
Q = (1.8) 1.9 - 2.4 (2.5) ; N = 20
Me = 4.6 × 2.2 µm ; Qe = 2.1

On overwintered White Oak -Quercus alba leaf/leaves tiny white cup fungi. Apothecia substipitate, about 0.15-0.32mm in diameter.
Asci 8-spored, H-, IKI+b.
Ascospores subfusiform, eguttulate, hyaline, measured
*5.6 - 6.2 (7.4) × (1.3) 1.4 - 1.6 (1.8) µm
Q = (3.5) 3.54 - 4.4 (4.5) ; N = 10
Me = 6 × 1.5 µm ; Qe = 4
Paraphyses lanceolate, without VBs.
Marginal hairs thick-walled, with crystals at the top, 1(-2) septate.

On dung, could be from turkey, tiny whitish cup fungi. Apothecia around 0.1mm, pulvinate, with asci sticking out.
Asci 8-spored, IKI-.
Ascospores subglobose to broadly ellipsoid, verrucose (large brown warts with some interconnections), measured
*(10.2) 10.3 - 11.4 (11.8) × (8.6) 8.8 - 9.4 (9.7) µm
Q = (1.1) 1.15 - 1.2 (1.3) ; N = 20
Me = 10.9 × 9.1 µm ; Qe = 1.2
Paraphyses spatulate or cylindrical with ovaloid VBs.
Collected on Jun 23 observed on Jul 11.

Habitat: on beargrass (Xerophyllum tenax) leaves near melting snow.
Ascocarps: dark burgundy, nearly black, sessile or with short, stout stalks, 0.7-1.3 mm tall, arising from a reddish-brown discoid, loosely attached subiculum. Stalk: 0-0.6 mm, capitula 0.5-0.8 high x 0.5-0.8 wide filled with a white spore mass. Asci: 8-spored, 85-125 x 11-12 um, with long thread-like tails. Peridium: yellow-brown, with a reticulated pattern. Cortex hyphae: septate, brown, no clamps, rounded tips, ~3.5-4.5 um diam. Spores: broadly elliptic, 10.2-12.3 x 8,5-10.2 µm, smooth.

On a piece of bark from a hardwood tree, small (0.3-0.4mm), black, superficial pseudothecia with black setae.
8-spored, bitunicate asci IKI-.
Hyaline spores with many septa, measure in H2O
(29.9) 31.3 - 43.6 (45.5) × (4.4) 4.5 - 5.35 (5.4) µm
Q = (5.6) 7.1 - 8.8 (9.2) ; N = 12
Me = 39 × 4.9 µm ; Qe = 8

this is a match C DNA
Cudoniella "sp-PA01"
Novel to all DNA repositories
DNA - ITS - Nanopore
voucher number now A-3049 dont know what happened
collectors V
https://www.inaturalist.org/observations/187333041

Tiny, under 0.5mm apothecia on decorticated hardwood stick, likely from Acer.
Ascospores measure in H2O
(8.7) 9.1 - 11.5 (12.6) × (2.5) 2.6 - 3 µm
Q = (3.2) 3.3 - 4 (4.5) ; N = 16
Me = 10.2 × 2.8 µm ; Qe = 3.6

Asci IKI+bb, croziers(-), measure in H2O
(44.2) 44.21 - 51.19 (51.2) × (6.6) 6.64 - 7.69 (7.7) µm
Q = (5.8) 5.82 - 7.7 ; N = 4
Me = 46.6 × 7.3 µm ; Qe = 6.4

Paraphyses cylindrical, septated, about 2um width, no VBs.

Tiny white substipitate discomycete on leaves. Apothecia with long marginal hairs around 100x3um with many septa.
Asci 8-spored, croziers(+), IKI-, 37-46x5.9-6.5um.
Ascospores are subfusiform to cylindrical, eguttulate, overmatured with one septum.
Paraphyses are filiform without VBs.

obtained in moist chamber from extremely rotten, wet wood of an old huge, long dead, Acer saccharum, sample was collected from this location on the 27 July 2022

Conidiomata localized, well defined sporodochia to very poorly defined, short stout synnemata, about 0.5mm tall at most, virtually identical to conidiomata produced by Hyalotrochophora lignatilis. Hypha. sinuous, thin, 0.6 - 0.8µm, appear to be sparsely branched and with few septa. Conidiophore probably undifferentiated from hyphae, conidiogenesis not observed but probably apical.

Conidia hyaline, coiled 1 1⁄2 time, with (5)7-8(9) septa, conidia are 12 - 15µm wide and individual filament are 3.5 - 5µm wide. A thin mucilaginous sheet appear to cover the conidia.

This species appear to be macroscopically very similar to Hyalotrochophora lignatilis, but easily differ by having conidia coiled 1 1⁄2 and not 1⁄2 (u shaped), with 5-9 septa (3 in H. lignatilis). Because Hyalotrochophora is monotypic, I had to look at possible option in other genera.

The species that I have found to be the most similar to this collection is Everhartia candida, described by Linder in is 1929 monograph of helicosporous hyphomycetous fungi. the protologue and illustration show a species that is extremely similar to this collection but with smaller conidia (9-12µ, filaments 3.3-5µ), the description also mention up to 7 septa in conidia (8 in the key), while several conidia in this collection had 8 septa and a few even 8 septa

The placement as Everhartia is only tentative, as E. hymenuloides, the type species might be an algae. Interestingly, the conidia of H. lignatilis also have a sheet and septa very similar to this species (see Flickr link below), and one have to wonder if the are related, especially considering the similar macromorphology, however more studies would be needed to verify this. Unfortunately, all my attempt to isolate this collection in pure culture failed, and thus for now I prefer to use the name Everhartia sp. for this collection

https://www.flickr.com/photos/31366379@N05/28569012783

@Keith_Seifert

A-3049
Collector: Cara Coulter

Spore Sizes:

(8.5)8.5-12.5 x (3.5)2.86-4.32 µm

38 spores measures from mature fresh specimen.

Specimen:
3.5cm to 2cm in length
Cap Size: .4-.6cm

Coloration:
Cream colored cap with gradual transition about halfway down the stipe to a darkened brown foot

Specimen found growing on log that was submerged in water. Half of the specimen's stipe was under water.

Habitat: Inundated sphagnum area with a mix of Hemlock, Birch, Maple and Rhododendron

On cedar small cup fungi. Apothecia white, 0.3-0.6mm in diameter with a short stipe.
Marginal hairs with crooks.
Asci 8-spored, IKI+bb Calycina type.
Ascospores hyaline, pyriform, measured
*(6.2) 6.5 - 8.1 (9.2) × (1.8) 2 - 2.4 (2.5) µm
Q = (2.9) 3 - 3.7 (3.9) ; N = 15
Me = 7.4 × 2.2 µm ; Qe = 3.3

With two hyphomycete on the same leaf.

Growing on a hardwood stick on the ground.
Tiny, apothecia about 1mm.
Asci 45-54um, IKI+b.
Paraphyses without vacuoles.
Spores smooth, hyaline, not septate, eguttulate, measure
(5.9) 6.5 - 8.7 (9.8) × (1.5) 1.6 - 2.1 (2.2) µm
Q = (3.4) 3.6 - 4.6 (5.4) ; N = 38
Me = 7.6 × 1.9 µm ; Qe = 4.1

White Lachnum sp. on plant litter and wood sticks up to an inch in diameter in a wet area.
-Hairs without oxalate crystals;
-Paraphyses without vacuoles, but very often with just one gluttule, 4.5-6um width, often wider than asci.
-Asci with croziers, 4-5 um width.
Ascospores hyaline, cylindrical, measure in H2O
(8) 8.3 - 9.9 (10.2) × (1.9) 2 - 2.3 (2.6) µm
Q = (3.6) 3.8 - 4.8 (5.5) ; N = 33
Me = 9.1 × 2.1 µm ; Qe = 4.3

On plant debris on the ground, under leaves. (Analyzed from leaves).
Apothecia 0.2-0.25mm.
Asci 8-spored with croziers, 38-46x4.4-5.6um
Paraphyses lanceolate, without vacuoles.
Marginal hairs 86-94x4.6-5um, septated with warts.

Found in my personal garden
Growing on dead, woody twigs of Malva assurgentiflora
Minute cups with a textured, greyish hymenophore, outer excipulum covered in dense white hairs. Many of the sporocarps have internal growth giving them a donut-like appearance

If interested in sphagnum bogs you can find many things simply by rinsing some sphagnum and checking the rinsate under the scope. I also like to use a moist chamber for this. All sorts of interesting things can be found in a tuft/mat of sphagnum. Rotifers, tartigrades and numerous fungal spores. Leave a tuft of moss in a moist chamber with a small amount of water at the bottom and examine the water over time. These Helicoon spores are quite distinctive. Helicoon pleuriseptatum Beverwijk from Sphagnum washes Oct and Nov 2021 Lake Verde bog and Black Ck (small patch of Sphagnum near slow rivulet).
Conidia 40-60µm diax20-30µm height, flattened, 6-10 coils/spirals, dark brown.
Mycoportal includes 22 specimens from the UK, US, NZ and Russia (Siberia). Most from bog habitats.

Collected during the 11th International Mycological Congress in San Juan, Puerto Rico. #imc11

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Image #1:
Canon EOS 6D + Canon MP-E 65mm f/2.8 1-5x + Yongnuo YN-14EX TTL LED Macro Ring Flash
1/180 sec, f/16, ISO 500
Color Corrected w/ X-Rite ColorChecker Passport
Image #2:
Canon EOS 6D + Canon MP-E 65mm f/2.8 1-5x + Yongnuo YN-14EX TTL LED Macro Ring Flash
1/180 sec, f/16, ISO 200
Color Corrected w/ X-Rite ColorChecker Passport
Image #3:
Canon EOS 6D + Canon MP-E 65mm f/2.8 1-5x + Yongnuo YN-14EX TTL LED Macro Ring Flash
1/180 sec, f/10, ISO 200
Color Corrected w/ X-Rite ColorChecker Passport
Image #4: Samsung Galaxy Note 4 + Dissecting Scope
Image #5:
100x – 3% KOH
Olympus CX30
Image #6:
400x – 3% KOH
Olympus CX30
Image #7:
1000x – 3% KOH
Olympus CX30
Image #8:
1000x – 3% KOH
Olympus CX30
Image #9:
1000x – 3% KOH
Olympus CX30
Image #10:
1000x – 3% KOH
Olympus CX30
Image #11:
1000x – 3% KOH
Olympus CX30
Image #12: habitat and host

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—MO custom fields—

Comments: see this species list for more observations of this fungus (or group of fungi), whose identity has evaded me for upwards of six years. in each example, the yellow stipe tissue turns deep purple in the presence of KOH. excited to not only to have a collection for sequencing and culturing, but for have received the provisional ID of Lindquistia — a xylariaceous anamorph — courtesy of IMA president, Dr. Keith Seifert, who I showed this to at the recent IMC in Puerto Rico. Dr. Seifert also possesses a portion of the collection, and I am working on gathering together more material from around the world.

Originally posted to Mushroom Observer on Jul. 27, 2018.

Permis de recherche scientifique Parc National d'Oka ;

PN0 2023-005

CG3045.
Érablière à caryer, chênes, hêtres. Au sol, grégaire.
Chapeau jusqu’à 4 cm. diam, viscidule.
Lames pourprées sur les jeunes spécimens, interveinées, sinuées.
Les lames pâlissent à maturité mais garde la couleur pourprée à l'arête.
Pied jaunâtre, strié, creux, longueur jusqu’à 6 cm.
Sporée blanche.
Récolté et déterminé par Yves Lamoureux.
Collectionné par Chantal Gauthier.

Non-stromatic, unitunicate, black pyrenomycetes fungi.
Small, hard perithecia up to 0.5 mm in diameter immersed in decorticated wood, with a well-developed neck. In a mixed forest.
Mature spores hyaline, with 3 septa, measure in H2O 25-30x5.9-6.4um

On deer dung tiny orange cup fungi with hyaline marginal hairs.
Asci 8-spored.
Ascospores ellipsoid, eguttulate, measured
*(18.7) 18.73 - 20.2 (20.4) × 9.3 - 10.3 (10.9) µm
Q = (1.8) 1.9 - 2 (2.1) ; N = 8
Me = 19.4 × 9.9 µm ; Qe = 2
Marginal hairs thick-walled, aseptate.

Growing abundantly on at least 3 well-rotted fallen hardwoods in close proximity. On the sides of the logs near where they meet the forest floor but not on the very bottoms. Salix nigra, Acer sacchariferum, Fagus grandifolia, Carya sp. and Carpinus caroliniana nearby as well as possibly some other hardwood species. Odor indistinct. Attached to wispy white rhizomorphs. Stems lateral. Caps finely fuzzy. Gills starting out pure white.

Small discomycete on decorticated hardwood next to Hormomyces aurantiacus. Apothecia up to 350um sessile.
8-spored asci with croziers, not sure about IKI, could be very weak blue, but strong blue after KOH. Asci measure in H2O
(43.2) 44.4 - 52.9 (54.4) × (6.7) 6.9 - 8.3 (8.6) µm
Q = (6.1) 6.13 - 6.5 (6.6) ; N = 6
Me = 48.7 × 7.7 µm ; Qe = 6.3
Ascospores ellipsoid, hyaline OCI=1, measure in H2O in asci
(7.2) 7.4 - 8.1 (8.3) × (2.8) 3.3 - 3.4 (3.8) µm
Q = (2) 2.1 - 2.4 (2.7) ; N = 6
Me = 7.7 × 3.3 µm ; Qe = 2.3
Paraphyses are cylindrical without VBs.
Marginal hairs smooth, slightly pigmented about 50-60x2.9-4.6um.
KOH negative.

Small cupulate fungi on Populus log. Apothecia are 0.4-0.7mm in diameter, sessile. Next to Scutellinia setosa.
Asci IKI+red, croziers(+).
Ascospores were still mostly in asci, hyaline, eguttulate, subfusiform, OCI=0-1, measure
(6.9) 7 - 9.3 (9.9) × (1.9) 2 - 2.5 (2.7) µm
Q = (2.6) 2.8 - 4.4 (4.6) ; N = 18
Me = 8.3 × 2.2 µm ; Qe = 3.7
Paraphyses are slightly lanceolate, without VBs.
Marginal hairs hyaline with many septa, 122x3.7um

I haven't seen this species before.

MRD0017

Station: Forêt d'Enseignement et de Recherche du Lac Duparquet

Lieu: Rapide-Danseur, Qc

Habitat : Forêt mixte (Sapin baumier et Peuplier Faux-tremble

Substrat : sous un tronc mort

Collectionneur : Mathias Rocheleau-Duplain

Déterminateur : Mathias Rocheleau-Duplain, Jonathan Mack et Jonathan Jensen-Lynch

MRD0040

1 janvier 2024
Pont-Rouge, Québec

Habitat : Corde de bois
Substrat : Buche d'Acer sp. (Érable)

Collectionneur : Mathias Rocheleau-Duplain

Taille du champignon : moins de 1 mm.

Mode de croissance : en colonie.